4/12/2023 0 Comments Inside out patch clamp methodThe sentence should begin: "Although some early, 'laborious' efforts." P. 1235, left column, last line: Quotation marks were misplaced. Note: In the version of this article initially published online: P. The protocol-from the beginning of slice preparation to the end of the first successful recording-can be completed in 3 h. Using this approach, all configurations of the patch-clamp technique (cell-attached, inside-out, whole-cell, outside-out and perforated patch) can be achieved, even for relatively distal dendrites, and simultaneous multiple-electrode dendritic recordings are also possible. Although the basic technique is similar to that used for somatic patching, we describe refinements and optimizations of slice quality, microscope optics, setup stability and electrode approach that are required for maximizing the success rate for dendritic recordings. This protocol describes techniques for recording from the dendrites of neurons in brain slices under direct visual control. Most patch-clamp recordings from neurons have been made from the soma, the largest structure of individual neurons, while their dendrites, which form the majority of the surface area and receive most of the synaptic input, have been relatively neglected. Abstract : The patch-clamp technique allows investigation of the electrical excitability of neurons and the functional properties and densities of ion channels.
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